Application

Research CategorySignaling

Research Sub CategorySignaling Neuroscience

This Anti-Modified Citrulline Antibody is validated for use in Western blotting and ELISA for the detection of Modified Citrulline.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Modified Citrulline in 10 µg of treated HL-60 cell lysate, using Anti-Human secondary antibody.
ELISA Analysis: A representative lot detected Modified Citrulline in ELISA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Citrulline is thought to be an unincorporated amino acid that plays a part in intermediary metabolism. It is produced in the small intestine by enterocytes. Citrulline concentration in plasma and serum is thought to be a reliable and noninvasive biomarker of small intestine function and mass during disease. Citrulline has also been shown to play an important role in protein homeostasis. Modified citrulline is a chemically modified form of citrulline that is more immunogenic.

Immunogen

Citrulline-containing peptide modified with 2,3-butanedione monoxime and antipyrine.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Human recombinant monoclonal IgG1λ in buffer containing PBS with 0.05% sodium azide.

Protein A purified

Format: Purified

Quality

Evaluated by Western Blotting in treated cornified human cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Modified Citrulline in 10 µg of treated cornified human cell lysate, using Anti-Human secondary antibody.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.